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pcmv flag myc n empty vector  (TaKaRa)


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    Structured Review

    TaKaRa pcmv flag myc n empty vector
    Pcmv Flag Myc N Empty Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv+flag+n+vector/pmc11279246-71-14-17
    Average 86 stars, based on 1 article reviews
    pcmv flag myc n empty vector - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Construct:

    Article Title: The N-terminal residual arginine 19 of influenza A virus NS1 protein is required for its nuclear localization and RNA binding.
    Article Snippet: RNA binding ability and cellular distribution are important for nonstructural protein 1 (NS1) of influenza A virus to act as a viral regulatory factor to control virus life cycle.. In this study, we identified that the N-terminal residues 19− 21 of NS1 are a highly conserved motif depending on all the available NS1 full length sequence of H5N1 influenza A virus from NCBI database.. Site-directed mutation analysis demonstrated that the R19 residue of NS1 is critical for its RNA binding and nuclear localization.

    Article Title: Mass Spectrometry-Based Proteomic Analysis of Potential Host Proteins Interacting with GP5 in PRRSV-Infected PAMs.
    Article Snippet: GP5, cloned from the PRRSV Hn07-1 genome, were constructed into pCMV-Myc-N vector (Clontech, Palo Alto, CA, USA, 631604). .. TAP1, TAP2, CANX, CALR, SLA-II, PSMB7, VPS16, and VPS18, cloned from the PAMs genome, were constructed into pCMV-Flag-N vector (Clontech, 635688). ..

    Article Title: Mass Spectrometry-Based Proteomic Analysis of Potential Host Proteins Interacting with GP5 in PRRSV-Infected PAMs
    Article Snippet: GP5, cloned from the PRRSV Hn07-1 genome, were constructed into pCMV-Myc-N vector (Clontech, Palo Alto, CA, USA, 631604). .. TAP1, TAP2, CANX, CALR, SLA-II, PSMB7, VPS16, and VPS18, cloned from the PAMs genome, were constructed into pCMV-Flag-N vector (Clontech, 635688). ..

    Plasmid Preparation:

    Article Title: The N-terminal residual arginine 19 of influenza A virus NS1 protein is required for its nuclear localization and RNA binding.
    Article Snippet: RNA binding ability and cellular distribution are important for nonstructural protein 1 (NS1) of influenza A virus to act as a viral regulatory factor to control virus life cycle.. In this study, we identified that the N-terminal residues 19− 21 of NS1 are a highly conserved motif depending on all the available NS1 full length sequence of H5N1 influenza A virus from NCBI database.. Site-directed mutation analysis demonstrated that the R19 residue of NS1 is critical for its RNA binding and nuclear localization.

    Article Title: PRRSV GP5 inhibits the antivirus effects of chaperone-mediated autophagy by targeting LAMP2A.
    Article Snippet: Endogenous microtubule-associated protein 1 light chain 3 (LC3), which was cloned from the PAM genome (NM_001170827), was ligated into the pEGFP-C3 vector (Clontech, 6082-1). .. GP3, GP4, GP5, M, and N, which were cloned from the PRRSV Hn07-1 Month XXXX Volume 0 Issue 0 10.1128/mbio.00532-24 3 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/m bi o on 0 5 Ju ly 2 02 4 by 2 40 3: 38 00 :3 28 3: a9 e: 88 39 :6 16 f: eb 58 :7 f2 . genome (KX766378), were inserted into the pCMV-Flag-N vector (Clontech, 635688). ..

    Article Title: TOLLIP inhibits the replication of PEDV by autophagic degradation of Nsp9.
    Article Snippet: Selective autophagy plays a crucial role in innate antiviral immunity by targeting essential viral components and host factors necessary for virus propagation.. Among these factors, the nonstructural protein 9 (Nsp9) of Porcine Epidemic Diarrhea Virus (PEDV) is required for viral replication.. However, the host factors regulating Nsp9 have remained elusive.

    Article Title: Mass Spectrometry-Based Proteomic Analysis of Potential Host Proteins Interacting with GP5 in PRRSV-Infected PAMs.
    Article Snippet: GP5, cloned from the PRRSV Hn07-1 genome, were constructed into pCMV-Myc-N vector (Clontech, Palo Alto, CA, USA, 631604). .. TAP1, TAP2, CANX, CALR, SLA-II, PSMB7, VPS16, and VPS18, cloned from the PAMs genome, were constructed into pCMV-Flag-N vector (Clontech, 635688). ..

    Article Title: PRRSV GP5 inhibits the antivirus effects of chaperone-mediated autophagy by targeting LAMP2A
    Article Snippet: Endogenous microtubule-associated protein 1 light chain 3 (LC3), which was cloned from the PAM genome (NM_001170827), was ligated into the pEGFP-C3 vector (Clontech, 6082-1). .. GP3, GP4, GP5, M, and N, which were cloned from the PRRSV Hn07-1 genome (KX766378), were inserted into the pCMV-Flag-N vector (Clontech, 635688). ..

    Article Title: Mass Spectrometry-Based Proteomic Analysis of Potential Host Proteins Interacting with GP5 in PRRSV-Infected PAMs
    Article Snippet: GP5, cloned from the PRRSV Hn07-1 genome, were constructed into pCMV-Myc-N vector (Clontech, Palo Alto, CA, USA, 631604). .. TAP1, TAP2, CANX, CALR, SLA-II, PSMB7, VPS16, and VPS18, cloned from the PAMs genome, were constructed into pCMV-Flag-N vector (Clontech, 635688). ..

    Amplification:

    Article Title: The N-terminal residual arginine 19 of influenza A virus NS1 protein is required for its nuclear localization and RNA binding.
    Article Snippet: RNA binding ability and cellular distribution are important for nonstructural protein 1 (NS1) of influenza A virus to act as a viral regulatory factor to control virus life cycle.. In this study, we identified that the N-terminal residues 19− 21 of NS1 are a highly conserved motif depending on all the available NS1 full length sequence of H5N1 influenza A virus from NCBI database.. Site-directed mutation analysis demonstrated that the R19 residue of NS1 is critical for its RNA binding and nuclear localization.

    Clone Assay:

    Article Title: PRRSV GP5 inhibits the antivirus effects of chaperone-mediated autophagy by targeting LAMP2A.
    Article Snippet: Endogenous microtubule-associated protein 1 light chain 3 (LC3), which was cloned from the PAM genome (NM_001170827), was ligated into the pEGFP-C3 vector (Clontech, 6082-1). .. GP3, GP4, GP5, M, and N, which were cloned from the PRRSV Hn07-1 Month XXXX Volume 0 Issue 0 10.1128/mbio.00532-24 3 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/m bi o on 0 5 Ju ly 2 02 4 by 2 40 3: 38 00 :3 28 3: a9 e: 88 39 :6 16 f: eb 58 :7 f2 . genome (KX766378), were inserted into the pCMV-Flag-N vector (Clontech, 635688). ..

    Article Title: TOLLIP inhibits the replication of PEDV by autophagic degradation of Nsp9.
    Article Snippet: Selective autophagy plays a crucial role in innate antiviral immunity by targeting essential viral components and host factors necessary for virus propagation.. Among these factors, the nonstructural protein 9 (Nsp9) of Porcine Epidemic Diarrhea Virus (PEDV) is required for viral replication.. However, the host factors regulating Nsp9 have remained elusive.

    Article Title: Mass Spectrometry-Based Proteomic Analysis of Potential Host Proteins Interacting with GP5 in PRRSV-Infected PAMs.
    Article Snippet: GP5, cloned from the PRRSV Hn07-1 genome, were constructed into pCMV-Myc-N vector (Clontech, Palo Alto, CA, USA, 631604). .. TAP1, TAP2, CANX, CALR, SLA-II, PSMB7, VPS16, and VPS18, cloned from the PAMs genome, were constructed into pCMV-Flag-N vector (Clontech, 635688). ..

    Article Title: PRRSV GP5 inhibits the antivirus effects of chaperone-mediated autophagy by targeting LAMP2A
    Article Snippet: Endogenous microtubule-associated protein 1 light chain 3 (LC3), which was cloned from the PAM genome (NM_001170827), was ligated into the pEGFP-C3 vector (Clontech, 6082-1). .. GP3, GP4, GP5, M, and N, which were cloned from the PRRSV Hn07-1 genome (KX766378), were inserted into the pCMV-Flag-N vector (Clontech, 635688). ..

    Article Title: Mass Spectrometry-Based Proteomic Analysis of Potential Host Proteins Interacting with GP5 in PRRSV-Infected PAMs
    Article Snippet: GP5, cloned from the PRRSV Hn07-1 genome, were constructed into pCMV-Myc-N vector (Clontech, Palo Alto, CA, USA, 631604). .. TAP1, TAP2, CANX, CALR, SLA-II, PSMB7, VPS16, and VPS18, cloned from the PAMs genome, were constructed into pCMV-Flag-N vector (Clontech, 635688). ..

    Article Title: Cytoplasmic Cargo Receptor p62 Inhibits Avibirnavirus Replication by Mediating Autophagic Degradation of Viral Protein VP2
    Article Snippet: .. 286 IBDV VP2 gene from segment A was cloned into the pCMV-Flag-N vector (635688; Clontech, 287 Mountain View, CA) and the pCMV-Myc-N vector (635689; Clontech, Mountain View, CA). ..

    other:




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